Figure 3: Immunohistochemical (A, B) and morphometric (C) analyses on the pancreas in different experimental groups. Sections in rat pancreas stained immunohistochemistry for insulin-positive β-cells (red), glucagon-positive α-cells (green), and nuclei (blue) of pancreatic islets in (A) untreated diabetic; (B) GS-treat diabetic rats. Magnification: 20X. Morphometric analysis of β-cell number (C) in pancreatic islets of diabetic and GS-treated rats. All values are expressed as mean ± SEM. Results represent the average from 6–8 rats with an average of 60–100 fields per rat. **P<0.01 versus diabetic group.