Figure 3: Photomicrographs of semithin sections of rats’ livers stained with toluidine blue, 1000X. A. The control group shows hepatocytes with vesicular nuclei (arrowheads) and blood sinusoids (S). Note a cytoplasmic vacuole within a hepatocyte (arrow). B. Group II reveals hepatocytes with vesicular nuclei (arrowheads) and the blood sinusoids (S). VonKupffer cell (thick arrow) and RBCs (curved arrow) are also seen. C. Group III shows hepatocytes with apparently small nuclei (arrowhead), multiple cytoplasmic vacuoles of variable sizes (arrows). A hepatocyte with karyorrhexed nucleus (curved arrow) and another with absent nucleus could be observed (double arrow). Note dilated blood sinusoids (S) and the endothelial cell (zigzag arrow) widely separated from the adjacent hepatocyte. D. Group IV demonstrates few hepatocytes with small nuclei (arrowhead); otherwise nuclei are comparable to control. The cytoplasm has few small vacuoles (arrow). Note blood sinusoids (S) apparently comparable to control.