Wrobel-Moustafa-Fixative Parafomaldehyde, 25% freshly prepared 40 ml Phosphate buffer (0.2 M, pH 7.4) 125 ml Saturated picric acid 37.5 ml Calcium chloride 5.0 mg Glutaraldehyde 25% 1.25 ml Add distilled water up to 250 ml

Table 2: Components of the fixative