Parameters Control subgroups Group II Group III Group IV   i ii iii iv v   AST level (IU/L) 34.30± 3.86 NS 36.4±4.57 NS 37.1±3.87 NS 38.6±4.19 NS 38.5±3.50 NS 98.2± 8.23aP 43.8±7.89 bP 45.4± 6.51 bP ALT level (IU/L) 165.5 ± 10.69 NS   171.9 ±11.81 NS 169.9 ±12.85 NS 173.4 ±12.56 NS 177.4 ±13.59 NS 315.2±12.86 aP 194.5±11.94 bP 198.3±14.12 bP ALP level (IU/L) 178.6 ± 12.01 NS   190.5 ±12.20 NS 185.7 ±11.47 NS 183.1 ±14.87 NS 190.5 ±11.46 NS 324.1±21.07 aP 186.2±10.16 bP 188.6±13.1bP MDA level(nmol/g tissue protein) 53.8 ±2.78 NS 51.8±3.19 NS 52.6 ± 3.77 NS 51.9 ±3.03 NS 51.7±2.98 NS 103.2±5.9 aP 60.9 ±5.25 bP 58.7 ±4.32 bP GSH level (µmol/g tissue protein) 6.54 ± 1.13 NS 7.09 ±0.65 NS 6.73 ±0.72 NS 7.11±0.80 NS 6.89 ±0.72 NS 2.35 ±0.77aP 7.99 ±0.98 bP 8.11 ±0.75 bP SOD level (units/mg tissue) 5.16± 0.47 NS 5.09 ±0.46 NS 5.19 ±0.56 NS 5.26 ±0.65 NS 5.31 ±0.47 NS 1.79 ± 0.4 aP 6.04 ±0.49 bP 5.89 ±0.64bP

Data is expressed as mean ± standard deviation,NS= non-significant difference between control subgroups tested by one-way ANOVA followed by Post Hoc Tukey’s test.P. value = probability of chance, P< 0.05 is significant tested by Student “t” test.aP = 0.01vs control group i;bP<0.01vs group II.ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; ALP: Alkalinephosphatase; MDA: Malondialdehyde; GSH; Glutathione; SOD: Superoxidedismutase.

Table 3: Serum levels of liver injury markers and measurement of some oxidative stress factors in the different groups studied.