Figure 2: Immunohistochemical detection of human LAM/TSC cells in uteri and anti-EGFR anitbody and rapamycin effect : detection of LAM/TSC cells by using COX IV rabbit monoclonal antibody (red staining) in uteri of mice 6 months after LAM/TSC cell administration (A), in LAM/TSC cell–administered mice treated with anti-EGFR antibody (B), and in LAM/TSC cell-administered mice treated with rapamycin (C) (X40). Scale bars: 50 μm. Quantification of LAM/TSC cells in uteri was calculated as percentage of COX IV-labelled LAM/ TSC cells on the total number of cells in the field (D). ***P<0.001, vs n° of COX IV labelled cells in LAM/TSC cell-administrated mice.