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Figure 3: Mycotoxin screening of various mold isolates cultured inYES broth using a multi-toxin thin-layer chromatography. Chromatograms eluted in toluene-ethyl acetate-formic acid (60+40+0.5) system (a) do not exhibit mycotoxins. Chromatograms eluted in toluene-ethyl acetate-formic acid (60+40+0.5) system (b) show the possible presence of ochratoxin (O) (arrow). Chromatograms eluted in toluene-ethyl acetate-formic acid (5+4+1) system (c) and in a hexane-ethyl acetate-acetic acid (18+3+1) system (d) fail to confirm the presence of ochratoxin. |