Table 3

Extraction conditions	EC₅₀ (µg/ml)	Reducing (OD₇₀₀)	Total phenol (g/g grape seed extracts)	Yield (%)
5 g grape seeds,3 hr.	286.67^g ± 3.07	1.6057^e ± 0.0381	0.2607ⁱ ± 0.0016	14.44^ab ± 0.03
7.5 g grape seed,3 hr.	271.33^e ± 1.76	1.8877^cd ± 0.0174	0.2660^g ± 0.0003	13.35^de ± 0.23
10g grape seed,3 hr.	260.67^cd ± 4.21	2.0307^b ± 0.0043	0.2680^f± 0.0006	12.98^ef ± 0.30
5 g grape seed,6 hr.	274.67^e ± 4.44	1.8450^d ± 0.1621	0.2633^h ± 0.0001	14.40^abc ± 0.13
7.5 g grape seed, 6 hr.	214.60^a ± 1.64	2.3847^a ± 0.0229	0.3286^a ± 0.0004	14.86^a ± 0.03
10 g grape seed, 6 hr.	252.67^b ± 1.56	2.3027^a ± 0.0572	0.2993^b ± 0.0004	12.67^fg ± 0.25
5 g grape seed,9 hr.	285.33^fg ± 4.84	1.7780^d± 0.0144	0.2673^fg ± 0.0007	14.16^bc ± 0.17
7.5 g grape seed, 9 hr.	274.00^e ± 3.01	1.8437^d ± 0.0230	0.2626^h ± 0.0010	14.54^ab ± 0.79
10 g grape seed, 9 hr.	258.67^c ± 3.01	2.3573^a ± 0.0218	0.2813^c ± 0.0013	12.98^ef ± 0.29
5 g grape seed,12 hr.	282.00^f± 4.34	2.0178^b ± 0.0317	0.2586^j ± 0.0002	14.81^ab± 0.06
7.5 g grape seed, 12 hr.	274.67^e ± 4.82	1.9917^bc ± 0.0304	0.2726^e ± 0.0007	13.75^cd ± 0.08
10 g grape seed, 12 hr.	263.00^d ± 1.51	2.1220^b ± 0.0395	0.2754^d ± 0.0007	12.20^f ± 0.05

Different letters indicate significant differences (P < 0.05) for values of one property down the column
Values are means ± SE (with 3 replications)

Table 3: The properties and yield of GSE using 50% ethanol at 50^°C, various amounts of grape seeds and extraction time.