Parameters Levels
Forward Extraction  
AOT 1 mM, 5 mM,10 mM,15 mM, 20 mM, 35mM
pH 6.0, 6.5, 7.0, 7.5, 8.0, 8.5
Time 30 min
Temperature 4°C
Backward Extraction  
Salt Concentration 1.5 M
pH 8.0
Time 1 hr
Temperature 4°C
No. of replicates = 3
No. of runs = 108
Table 1: Experimental parameters.