Parameters SA RM
AE (Unit/mL) 0.22 ± 0.01 0.48 ± 0.01
TA (Unit) 1.10 ± 0.05 2.39 ± 0.05
Cp (μg/mL) 41.64 ± 5.48 31.87 ± 1.31
SA (Unit/mg) 5.31 ± 0.19 14.98 ± 0.49
PF 11.08 ± 0.39 31.27 ± 1.02
RY (%) 46.72 ± 2.23 100.85 ± 2.23
Forward extraction: AOT concentration 20 mM, pH 7.0
Backward extraction: KCl concentration 1.5 M, pH 7.5
*Sample size: 5 mL
Table 6: Effect of methods on chymotrypsin extraction parameters.