Table 4

Purification Method	A_E (U)	C_P (mg)	SA (U/mg)	PF	RY (%)
ASF	30.66±1.84	12.02±0.32	2.55±0.14	2.46±0.14	70.4±4.23
ATPS	37.67±0.38	6.98±0.12	5.40±0.09	5.20±0.08	86.6±0.88
P-value^*	0.023	0.002	0.000	0.000	0.023

^*P-values were obtained from the t-test by comparing A_E, C_P, SA, PF and RY of the two methods, respectively
A_E: enzyme activity
C_P: protein content
SA: specific activity (A_E / C_P)
K_P: partition coefficient (C_P of top phase / C_P of bottom phase)
PF: purification factor (SA of purified enzyme / SA of crude enzyme)
RY: recovery yield (A_E of purified enzyme / A_E of crude enzyme)

Table 4: ASF and ATPS comparison.