Figure 1: DEAE-cellulose chromatography for purification of PSAO. 5 milliliters solution containing 30 mgmL-1 protein was applied to a DEAE-cellulose column (Ø= 2.5 cm, l = 20 cm) equilibrated with KPi buffer (0.02 M, pH 7.0) at a flow rate of 15 mL/h. Arrow indicates start of elution by KPi buffer (0.1 M, pH 7.0) containing 0.1 M KCl. 3 milliliters were collected in each tube. Active fractions - tubes 9-21 - were pooled and lyophilized.