Figure 2: Electrophoretic analysis of PSAO on different stages of its purification. Proteins (2 mg mL-1) at each purification step were resolved by SDS-PAGE and stained with Coomassie Brilliant Blue. Line 1: molecular mass markers, lane 2: Sephadex G-200 chromatography, line 3: DEAE-cellulose chromatography, line 4: 70% ammonium sulfate fractionation, line 5: 30% ammonium sulfate fractionation and line 6: filtrate of pea seedling homogenate.