Free Amino Acid TSp D-TSp TSa D-TSa VC SC Essential             Histidine 1.32 ± 0.33 1.86 ± 0.55 1.47 ± 0.21 3.41 ± 1.19 3.78 ± 0.27 3.09 ± 1.01 Isoleucine 2.70 ± 0.08 2.78 ± 0.28 3.78 ± 0.40 3.68 ± 0.09 4.03 ± 0.28 3.37 ± 0.13 Leucine 7.05 ± 0.49 6.97 ± 0.77 8.28 ± 0.30 7.92 ± 0.19 11.65 ± 0.27 9.88 ± 0.31 Lysine 7.46 ± 0.24 10.99 ± 0.50 7.49 ± 0.46 10.49 ± 0.74 11.45 ± 0.58 9.69 ± 0.24 Methionine n.a. n.a. n.a. n.a. n.a. n.a. Phenylalanine 2.86 ± 0.01 3.89 ± 0.38 3.14 ± 0.03 4.34 ± 0.61 4.85 ± 0.39 4.77 ± 0.33 Threonine 3.78± 0.47 5.46 ± 0.40 4.59 ± 0.12 5.72 ± 0.53 6.02 ± 0.27 4.46 ± 0.22 Tryptophan n.a. n.a. n.a. n.a. n.a. n.a. Valine 4.29 ± 0.54 3.16 ± 0.10 6.32 ± 0.69 3.98 ± 0.59 2.94 ± 0.39 5.37 ± 0.08 Total (a) 29.54 35.19 35.05 39.54 44.72 40.63 Nonessential Alanine 7.92 ± 0.31 2.88 ± 1.38 10.61 ± 0.38 4.88 ± 0.88 7.43 ± 0.74 12.14 ± 0.44 Arginine 38.13 ± 1.98 29.45 ± 1.00 25.64 ± 2.00 20.63 ± 0.08 10.65 ± 0.82 18.47 ± 0.31 Aspartic acid & Asparagine 4.41 ± 0.24 8.80 ± 0.26 5.91 ± 0.07 8.23 ± 0.00 6.91 ± 0.40 5.91 ± 0.35 Cysteine n.a. n.a. n.a. n.a. n.a. n.a. Glutamic acid & Glutamine 5.72 ± 0.27 9.87 ± 0.87 8.25 ± 0.35 10.64 ± 0.15 12.70 ± 0.56 5.61 ± 0.20 Glycine 6.11 ± 0.92 2.88 ± 0.68 5.95 ± 0.46 4.52 ± 0.26 6.97 ± 0.06 10.28 ± 0.45 Proline 1.80 ± 0.01 1.94 ± 0.00 1.90 ± 0.10 1.50 ± 0.01 1.92 ± 0.19 1.35 ± 0.02 Serine 4.77 ± 0.88 6.59 ± 0.37 4.96 ± 0.19 7.44 ± 0.39 5.97 ± 0.10 3.58 ± 0.13 Tyrosine 1.59 ± 0.01 2.40 ± 0.31 1.73 ± 0.09 2.61 ± 0.27 2.72 ± 0.31 2.03 ± 0.08 Total (b) 70.46 64.81 64.95 60.46 55.28 59.37 Grand total (a + b) 100 100 100 100 100 100 Grand total (g/L) 0.40 ± 0.02 0.09 ± 0.02 0.48 ± 0.01 0.11 ± 0.00 0.02 ± 0.00 0.10 ± 0.01 Values are in % of total free amino acids (last row) in the brines and given as means (n = 3) ± standard deviations (absolute values). n.a. not analyzed.

Table 5: Free amino acid profile of the six different brines.