Figure 3: HPLC profiles of nano-encapsulated pentapeptide stored at 0 (left) and 60 (right) days. DMSO peak is shown on the graph (retention time 1.8 min). Solvent system: 0.1% TFA in water and 0.1%TFA in water and acetonitrile (50:50); flow rate: 1 mL/min; injection volume: 10 μL; elution time: 45 min; absorbance measured.