| |
Listeria monocytogenes |
| Cells concentration |
6 log CFU mL-1 |
6 log CFU mL-1 |
6 log CFU mL-1 |
4 log CFU mL-1 |
4 log CFU mL-1 |
4 log CFU mL-1 |
2 log CFU mL-1 |
2 log CFU mL-1 |
2 log CFU mL-1 |
| MIC/MBC |
MIC 24h (mg L-1) |
MIC 48h (mg L-1) |
MBC (mg L-1) |
MIC 24h (mg L-1) |
MIC 48h (mg L-1) |
MBC (mg L-1) |
MIC 24h (mg L-1) |
MIC 48h (mg L-1) |
MBC (mg L-1) |
| Citral |
350 |
475 |
500 |
325 |
425 |
425 |
250 |
300 |
325 |
| 2-(E)-hexenal |
1250 |
1400 |
>1500 |
1250 |
1400 |
>1500 |
850 |
1300 |
1400 |
| Hexanal |
>1500 |
>1500 |
>1500 |
1500 |
>1500 |
>1500 |
1350 |
>1500 |
>1500 |
| Citron oil |
>1200 |
>1200 |
>1200 |
>1000 |
>1000 |
>1000 |
500 |
>1000 |
>1000 |
| Carvacrol |
175 |
200 |
225 |
150 |
175 |
200 |
100 |
175 |
200 |
|
| |
Escherichia coli |
| Cells concentration |
6 log CFU mL-1 |
6 log CFU mL-1 |
6 log CFU mL-1 |
4 log CFU mL-1 |
4 log CFU mL-1 |
4 log CFU mL-1 |
2 log CFU mL-1 |
2 log CFU mL-1 |
2 log CFU mL-1 |
| MIC/MBC |
MIC 24h (mg L-1) |
MIC 48h (mg L-1) |
MBC (mg L-1) |
MIC 24h (mg L-1) |
MIC 48h (mg L-1) |
MBC (mg L-1) |
MIC 24h (mg L-1) |
MIC 48h (mg L-1) |
MBC (mg L-1) |
| Citral |
>1500 |
>1500 |
>1500 |
>1500 |
>1500 |
>1500 |
>1500 |
>1500 |
>1500 |
| 2-(E)-hexenal |
525 |
600 |
650 |
500 |
575 |
575 |
500 |
525 |
525 |
| Hexanal |
>1500 |
>1500 |
>1500 |
1050 |
>1500 |
>1500 |
700 |
1200 |
>1200 |
| Citron oil |
>1200 |
>1200 |
>1200 |
>1200 |
>1200 |
>1200 |
>1000 |
>1000 |
>1000 |
| Carvacrol |
200 |
200 |
225 |
200 |
200 |
200 |
200 |
200 |
200 |
|
| |
Salmonella Enteritidis |
| Cells concentration |
6 log CFU mL-1 |
6 log CFU mL-1 |
6 log CFU mL-1 |
4 log CFU mL-1 |
4 log CFU mL-1 |
4 log CFU mL-1 |
2 log CFU mL-1 |
2 log CFU mL-1 |
2 log CFU mL-1 |
| MIC/MBC |
MIC 24h (mg L-1) |
MIC 48h (mg L-1) |
MBC (mg L-1) |
MIC 24h (mg L-1) |
MIC 48h (mg L-1) |
MBC (mg L-1) |
MIC 24h (mg L-1) |
MIC 48h (mg L-1) |
MBC (mg L-1) |
| Citral |
>1500 |
>1500 |
>1500 |
>1500 |
>1500 |
>1500 |
>1500 |
>1500 |
>1500 |
| 2-(E)-hexenal |
>1500 |
>1500 |
>1500 |
>1500 |
>1500 |
>1500 |
800 |
1300 |
1500 |
| Hexanal |
>1500 |
>1500 |
>1500 |
1050 |
>1500 |
>1500 |
>1500 |
>1500 |
>1500 |
| Citron oil |
>1200 |
>1200 |
>1200 |
>1200 |
>1200 |
>1200 |
>1000 |
>1000 |
>1000 |
| Carvacrol |
200 |
200 |
250 |
175 |
175 |
200 |
175 |
175 |
200 |
|
| |
Bacillus cereus |
| Cells concentration |
6 log CFU mL-1 |
6 log CFU mL-1 |
6 log CFU mL-1 |
4 log CFU mL-1 |
4 log CFU mL-1 |
4 log CFU mL-1 |
2 log CFU mL-1 |
2 log CFU mL-1 |
2 log CFU mL-1 |
| MIC/MBC |
MIC 24h (mg L-1) |
MIC 48h (mg L-1) |
MBC (mg L-1) |
MIC 24h (mg L-1) |
MIC 48h (mg L-1) |
MBC (mg L-1) |
MIC 24h (mg L-1) |
MIC 48h (mg L-1) |
MBC (mg L-1) |
| Citral |
300 |
>650 |
>650 |
300 |
350 |
350 |
300 |
300 |
300 |
| 2-(E)-hexenal |
1200 |
1350 |
>1400 |
1200 |
1350 |
>1400 |
800 |
1250 |
1300 |
| Hexanal |
>1500 |
>1500 |
>1500 |
1500 |
>1500 |
>1500 |
1350 |
>1500 |
>1500 |
| Citron oil |
>2000 |
>2000 |
>2000 |
>2000 |
>2000 |
>2000 |
>2000 |
>2000 |
>2000 |
| Carvacrol |
175 |
175 |
200 |
150 |
175 |
175 |
150 |
150 |
150 |
|
| |
Staphylococcus aureus |
| Cells concentration |
6 log CFU mL-1 |
6 log CFU mL-1 |
6 log CFU mL-1 |
4 log CFU mL-1 |
4 log CFU mL-1 |
4 log CFU mL-1 |
2 log CFU mL-1 |
2 log CFU mL-1 |
2 log CFU mL-1 |
| MIC/MBC |
MIC 24h (mg L-1) |
MIC 48h (mg L-1) |
MBC (mg L-1) |
MIC 24h (mg L-1) |
MIC 48h (mg L-1) |
MBC (mg L-1) |
MIC 24h (mg L-1) |
MIC 48h (mg L-1) |
MBC (mg L-1) |
| Citral |
500 |
550 |
550 |
450 |
500 |
500 |
250 |
250 |
250 |
| 2-(E)-hexenal |
1200 |
1400 |
>1500 |
1300 |
1400 |
>1500 |
900 |
1300 |
1400 |
| Hexanal |
>1500 |
>1500 |
>1500 |
1500 |
>1500 |
>1500 |
1400 |
>1500 |
>1500 |
| Citron oil |
>1200 |
>1200 |
>1200 |
>1000 |
>1000 |
>1000 |
800 |
>1000 |
>1000 |
| Carvacrol |
275 |
275 |
275 |
200 |
250 |
250 |
150 |
200 |
200 |
|
| Table 1: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of citral, 2-(E)-hexenal, hexanal, citron oil and carvacrol against L.
monocytogenes, E. coli, S. enteritidis, B. cereus and S. aureus in relation to the inoculum level. |
