Figure 1: Anion exchange chromatography of the crude GMP on a 1.5 × 5.7 cm column of DEAE-Sephacel. (A): Fractions collected were monitored for UV absorbance and sialic acid. (B): Fractions collected were also monitored for galactose, galactosamine, and phosphorus. Horizontal bars denote the fractions that were pooled for further study.