Figure 2: Size exclusion HPLC on Superdex-75. (A): Elution pattens for the crude GMP, purified GMP, and unadsorbed product are shown. The purified GMP had a small peak eluting at ˜43 min shown by an arrow. (B): The chromatogram shows elution patterns of molecular size markers including: (1) bovine serum albumin (67 kDa); (2) β-lactoblobulin (36.6 kDa); (3) cytochrome-C (13.6 kDa); (4) aprotinin (6.512 kDa); and (5) vitamin B12 (1.355 kDa).