| |
Crude GMP |
Product adsorbed on the column (purified GMP) |
Product unadsorbedon the column |
| Asx |
0.50a |
0.47 ± 0.05b |
0.62 ± 0.04b |
| Glx |
1.10 |
1.05 ± 0.08 |
1.34 ± 0.10 |
| Ser |
0.66 |
0.57 ± 0.08 |
0.72 ± 0.10 |
| Gly |
0.12 |
0.11 ± 0.01 |
0.13 ± 0.01 |
| Thr |
1.17 |
1.17 ± 0.10 |
1.28 ± 0.07 |
| Arg |
0.02 |
0.01 ± 0.00 |
0.03 ± 0.01 |
| Ala |
0.53 |
0.52 ± 0.04 |
0.74 ± 0.25 |
| Val |
0.50 |
0.54 ± 0.02 |
0.64 ± 0.05 |
| Ile |
0.58 |
0.60 ± 0.04 |
0.69 ± 0.08 |
| Leu |
0.15 |
0.12 ± 0.01 |
0.24 ± 0.03 |
| Lys |
0.27 |
0.29 ± 0.02 |
0.40 ± 0.02 |
| Tyr |
0.06 |
nd |
0.02 ± 0.02 |
| Phe |
0.03 |
nd |
0.04 ± 0.01 |
| Trp |
< 0.01 |
< 0.01 |
< 0.01 |
| Absorbance at 280 nm for 1 mg/mL solutionc |
0.061 ± 0.00 |
0.014 ± 0.001 |
0.072 ± 0.030 |
|
Proline, methionine, and cysteine were not determined.
aAverage of two determinations.
bMean ± SD (n = 6).
nd: Not detected.
cEach sample was filtered through 0.22 μm membrane before measurement of
dbsorbance, and absorvance values are shown as mean ± SD (n = 6). |
| Table 1: Amino acid concentrations (μmole/mg) and UV absorbance determined
in the crude GMP and its fractions obtained by anion exchange chromatography
(Figure 1). |
