Crude GMP Product adsorbed on the column (purified GMP) Product unadsorbedon the column
Asx 0.50a 0.47 ± 0.05b 0.62 ± 0.04b
Glx 1.10 1.05 ± 0.08 1.34 ± 0.10
Ser 0.66 0.57 ± 0.08 0.72 ± 0.10
Gly 0.12 0.11 ± 0.01 0.13 ± 0.01
Thr 1.17 1.17 ± 0.10 1.28 ± 0.07
Arg 0.02 0.01 ± 0.00 0.03 ± 0.01
Ala 0.53 0.52 ± 0.04 0.74 ± 0.25
Val 0.50 0.54 ± 0.02 0.64 ± 0.05
Ile 0.58 0.60 ± 0.04 0.69 ± 0.08
Leu 0.15 0.12 ± 0.01 0.24 ± 0.03
Lys 0.27 0.29 ± 0.02 0.40 ± 0.02
Tyr 0.06 nd 0.02 ± 0.02
Phe 0.03 nd 0.04 ± 0.01
Trp < 0.01 < 0.01 < 0.01
Absorbance at 280 nm for 1 mg/mL solutionc 0.061 ± 0.00 0.014 ± 0.001 0.072 ± 0.030
Proline, methionine, and cysteine were not determined.
aAverage of two determinations.
bMean ± SD (n = 6).
nd: Not detected.
cEach sample was filtered through 0.22 μm membrane before measurement of
dbsorbance, and absorvance values are shown as mean ± SD (n = 6).
Table 1: Amino acid concentrations (μmole/mg) and UV absorbance determined in the crude GMP and its fractions obtained by anion exchange chromatography (Figure 1).