Food aliquot pH Added water Added phytase (per 100g of food) 1 Native (6.8) Nil 5000 FTU 2 5.1 Nil 5000 FTU 3 5.1 100% w/w 5000 FTU

FTU, phytase units defined as amount of enzyme that liberates 1 μmol of inorganic phosphorus per minute

Table 2: Treatments for phytase study.