Sample Strain weeks Lactic acid (mg/l) Acetic acid (mg/l) Citric Acid (mg/l) Malic acid (mg/l) Ascorbic acid (mg/l)
Juice (pH adjusted to 4.0 by tri-sodium citrate) L. plantarum 0 387 ± 4.92 119 ± 1.01 3318 ± 24 29966 ±154 299 ± 2.22
  1 413 ± 5.79 122 ± 2.09 2913 ± 19 26077± 123a 298 ± 3.10
  2 429 ± 4.70a 134 ± 2.00a 2305 ± 21a 24822±129a 297 ± 2.14
  3 432 ± 5.36a 165 ± 2.12a 2140 ± 22a 20429±132a 298 ± 2.01
  4 460 ± 6.45a 192 ± 2.42a 1907 ± 32a 16378±144a 296 ± 2.28
             
L. rhamnosus 0 391 ± 4.10 115 ± 3.31 3215 ± 27 30919±186 299 ± 1.17
  1 412 ± 5.42 122 ± 2.05 2962 ± 23 27632±165a 298 ± 2.05
  2 425 ± 4.83a 139 ± 4.13a 2437 ± 33a 24184±210a 297 ± 3.21
  3 439 ± 6.00a 167 ± 3.07a 2087 ± 22a 19546±184a 298 ± 2.12
  4 453 ± 5.06a 194 ± 5.10a 1927 ± 26a 15339±0.01a 298 ± 2.18
Juice containing WPC L. plantarum 0 398 ± 7 118 ± 4 3342 ± 34 30240±219 297 ± 2.14
  1 417 ± 9 150 ± 3 3067 ± 11 26720±113a 296 ± 3.15
  2 439 ± 6a 169 ± 8a 2318 ± 19a 25990±104a 298 ± 3.18
  3 452± 5a 199 ± 5a 2107 ± 32a 20110±134a 298 ± 3.17
  4 461 ± 4a 217 ± 7a 2098 ± 22a 12390±101a 297 ± 3.16
             
L. rhamnosus 0 380 ± 5 110 ± 8 3229 ± 26 30660±168 298 ± 2.25
  1 415 ± 4 143 ± 5 2915 ± 42 26650±149a 298 ± 3.13
  2 447 ± 8a 167 ± 4a 2514 ± 15a 22180±125a 297 ±2.21
  3 453 ± 6a 205 ± 8a 2287 ± 43a 20160±119a 297 ± 2.18
  4 467 ± 7a 212 ± 9a 2049 ± 28a 15250±115a 295 ± 2.14
The data presented in this table are means of three replicates ± standard deviation. aThe mean values show statistically significant differences at p≤0.001 according to the post hoc comparisons (Tukey’s test) of the ANOVA. The data of organic acids at different time intervals were compared against day zero time point
Table 2: Organic acids content in probiotic fortified seabuckthorn juice samples during 4 weeks of cold storage.