Employed Solution Time Fix Solution 10% ethanol, 0.5% acetic acid 10 min Silver Stainig 0.2% silver nitrate in fix solution 5 min Washing (twice) bi-distilled water 20 s and 2 min Developer Solution 3% NaOH, 0.5% formaldehyde ~5 min Until bands appears Fix Solution 10% ethanol, 0.5% acetic acid 5 min Washing bi-distilled water 5 min a. Slightly modified from Sanguinetti et al. [4].

Table 1: Rapid silver staining protocola employed for STR detection.