Table 3

Sample	Sample Dilution (ng/µL)	SRY			RPPH1			IPC
		Mean Quantity (ng/µL)	Std Dev	95% Confidence (± percent)	Mean Quantity (ng/µL)	Std Dev	95% Confidence (± percent)	C_T	Std Dev	95% Confidence (± percent)
A	20	20.10	1.051	10.46	21.15	0.804	7.60	29.70	0.037	0.25
	10	8.98	0.400	8.91	9.11	0.341	7.49	29.73	0.034	0.23
	1	0.85	0.109	25.65	0.87	0.051	11.72	29.92	0.034	0.23
	0.10	0.08	0.007	17.50	0.09	0.014	31.11	29.97	0.039	0.26
	0.05	0.05	0.028	112.00	0.04	0.002	10.00	30.00	0.032	0.21

B	20	23.09	2.219	19.22	24.36	1.656	13.60	29.79	0.038	0.26
	10	11.22	0.485	8.65	11.49	0.529	9.21	29.77	0.046	0.31
	1	1.15	0.142	24.70	1.14	0.083	14.56	29.89	0.030	0.20
	0.10	0.11	0.013	23.64	0.10	0.008	16.00	29.98	0.040	0.27
	0.05	0.05	0.015	60.00	0.06	0.007	23.33	29.98	0.012	0.08

C	20	23.11	0.821	7.11	22.51	0.294	2.61	29.62	0.055	0.37
	10	9.25	0.601	12.99	8.72	0.562	12.89	29.67	0.053	0.36
	1	0.89	0.039	8.76	0.82	0.027	6.59	29.81	0.057	0.38
	0.10	0.11	0.027	49.09	0.10	0.008	16.00	29.89	0.053	0.35
	0.05	0.04	0.020	100.00	0.04	0.008	40.00	29.81	0.047	0.32

D	20	26.49	2.116	15.98	27.28	1.835	13.45	29.90	0.106	0.71
	10	13.09	0.596	9.11	13.26	0.261	3.94	29.87	0.028	0.19
	1	1.26	0.136	21.59	1.22	0.081	13.28	29.76	0.055	0.37
	0.10	0.12	0.032	53.33	0.12	0.008	13.33	30.02	0.034	0.23
	0.05	0.07	0.021	60.00	0.06	0.005	16.67	29.97	0.042	0.28

E	20	female	----	----	24.91	0.586	4.70	29.97	0.023	0.15
	10	female	----	----	12.11	0.486	8.03	29.87	0.007	0.05
	1	female	----	----	1.14	0.049	8.60	29.77	0.018	0.12
	0.10	female	----	----	0.12	0.016	26.67	29.73	0.089	0.60
	0.05	female	----	----	0.06	0.008	26.67	29.71	0.047	0.32

Reproduced with permission from Reference 12; the values for 95% confidence are recalculated.

Table 3: Reproducibility study: measured and expected quantities, standard deviations and 95% confidence intervals for four male and one female DNA samples across three consecutive runs.