Sample Sample
Dilution
(ng/µL)
SRY RPPH1 IPC
    Mean
Quantity
(ng/µL)
Std Dev 95%
Confidence
( percent)
Mean
Quantity
(ng/µL)
Std Dev 95%
Confidence
(± percent)
CT Std Dev 95%
Confidence
(± percent)

A

20

20.10

1.051

10.46

21.15

0.804

7.60

29.70

0.037

0.25

 

10

8.98

0.400

8.91

9.11

0.341

7.49

29.73

0.034

0.23

 

1

0.85

0.109

25.65

0.87

0.051

11.72

29.92

0.034

0.23

 

0.10

0.08

0.007

17.50

0.09

0.014

31.11

29.97

0.039

0.26

 

0.05

0.05

0.028

112.00

0.04

0.002

10.00

30.00

0.032

0.21

                     

B

20

23.09

2.219

19.22

24.36

1.656

13.60

29.79

0.038

0.26

 

10

11.22

0.485

8.65

11.49

0.529

9.21

29.77

0.046

0.31

 

1

1.15

0.142

24.70

1.14

0.083

14.56

29.89

0.030

0.20

 

0.10

0.11

0.013

23.64

0.10

0.008

16.00

29.98

0.040

0.27

 

0.05

0.05

0.015

60.00

0.06

0.007

23.33

29.98

0.012

0.08

                     

C

20

23.11

0.821

7.11

22.51

0.294

2.61

29.62

0.055

0.37

 

10

9.25

0.601

12.99

8.72

0.562

12.89

29.67

0.053

0.36

 

1

0.89

0.039

8.76

0.82

0.027

6.59

29.81

0.057

0.38

 

0.10

0.11

0.027

49.09

0.10

0.008

16.00

29.89

0.053

0.35

 

0.05

0.04

0.020

100.00

0.04

0.008

40.00

29.81

0.047

0.32

                     

D

20

26.49

2.116

15.98

27.28

1.835

13.45

29.90

0.106

0.71

 

10

13.09

0.596

9.11

13.26

0.261

3.94

29.87

0.028

0.19

 

1

1.26

0.136

21.59

1.22

0.081

13.28

29.76

0.055

0.37

 

0.10

0.12

0.032

53.33

0.12

0.008

13.33

30.02

0.034

0.23

 

0.05

0.07

0.021

60.00

0.06

0.005

16.67

29.97

0.042

0.28

                     

E

20

female

 ----

 ----

24.91

0.586

4.70

29.97

0.023

0.15

 

10

female

 ----

 ----

12.11

0.486

8.03

29.87

0.007

0.05

 

1

female

 ----

 ----

1.14

0.049

8.60

29.77

0.018

0.12

 

0.10

female

 ----

 ----

0.12

0.016

26.67

29.73

0.089

0.60

 

0.05

female

 ----

 ----

0.06

0.008

26.67

29.71

0.047

0.32

Reproduced with permission from Reference 12; the values for 95% confidence are recalculated.
Table 3: Reproducibility study: measured and expected quantities, standard deviations and 95% confidence intervals for four male and one female DNA samples across three consecutive runs.