Organism

# clones sequenced (expired lot)a

PCR (expired lot)b

# clones sequenced (new lot)

PCR (new lot)

Acinetobacter baumannii CP001172

5

+

0

-

Bacillus cereus CP000227

3

+

2

+

Bacillus licheniformis EF685205

15

+

58

+

Enterococcus faecium GQ405286

94

+

0

-

Brevibacillus choshinensis AB038650

0

+

2

+

Bacillus subtilis

0

-

38

+


a117 cpn60 isolates from the expired Product A and 100 cpn60 isolates from the new lot were sequenced. Bacterial sequences were determined by BLAST analysis using NCBI or against the cpn60 database http://www.cpndb.ca/cpnDB/home.php) (Hill et al, 2004)
bPCR represents a positive signal against total extracted consortia DNA using cpn60 primers specific for that species
Table 5: Consortia composition by cpn60 isolate sequencing.