Antibody

Maker

Clone

Species

AG-retrieval

incubation

AB-dilution

CB

EPITOMICS 

EPR4323:

R

autoclave

overnight

1:400

CD

santa cruz

E-7

M

autoclave

overnight

1:200

eNOS

 Gene Tex

polyclonal:

R

autoclave

overnight

prediluted

GC-R

Novus

BuGR2:

M

autoclave

overnight

1:400

MUC 4

abcam

8G-7

M

autoclave

overnight

1:400

SIRT1

Novus

E104:

R

autoclave

overnight

1:400

HSP70

santa cruz

polyclonal:

G

autoclave

overnight

1:400

P53

santa cruz

FL-393:

G

autoclave

overnight

1:400

HCK

Nichirei

34βE12:

M

retrievalsolution

overnight

prediluted

VEGF

Milipore

JH121

M

autoclave

overnight

1:400

CD15

Dako

C3D-1:

M

non

overnight

1:200

Ley

Dako

BM-1:

M

non

overnight

1:200

Lea

Calbiochem

T174

M

non

overnight

1:200

Leb

Calbiochem

T218

M

non

overnight

1:200

A

Ortho

 

M

non

overnight

1:400

B

Ortho

 

M

non

overnight

1:400

H

MONOSAN

A-70/A9

M

non

overnight

1:100

Table 1: Characteristics of the antibodies used.