Figure 2: Alutetraplexes utilizing crude DNA preparations stored for several years with several freeze thaws, analyzed on 2% agarose and stained with GelRed. 2A. Alutetraplex 1. 2B. Alutetraplex 2. Lanes: M refers to Benchtop pGEM DNA Markers (Promega, Madison, WI); numbers indicated on top of the wells designate the individuals in the pedigree shown in Figure 4.