S. No Constituents Rf values Mean of Rf values Range of variation of Rf values
1st 2nd 3rd
1 Cannabinol 0.308 0.310 0.318 0.313 -0.004 to 0.006
2 Cannabidiol 0.955 0.48 0.951 0.952 -0.003 to 0.004
3 Tetrahydrocannabinol 0.984 0.981 0.980 0.982 -0.002 to 0.002
Table 4: Range of variation of Rf values for different compound in ethyl acetate, methanol and ammonia.