| Sample ID |
Dilution |
Absorbance of Dilution |
Estimated Conc. of Dilution (ppm) |
Conc. of the extract |
Average Concentration of each donor |
| PSU-01a |
1:2 |
0.8090 |
2.2490 |
4.4980 |
5.2875 |
| PSU-01b |
1:2 |
1.5844 |
3.7467 |
7.4934 |
|
| PSU-01c |
1:2 |
0.6468 |
1.9356 |
3.8712 |
|
| PSU-02a |
1:10 |
0.2942 |
1.2693 |
12.6933 |
20.6532 |
| PSU-02b |
1:10 |
1.1577 |
2.9567 |
29.5673 |
|
| PSU-02c |
1:10 |
0.6527 |
1.9699 |
19.6990 |
|
| PSU-03a |
1:10 |
0.6867 |
2.0363 |
20.3632 |
12.0047 |
| PSU-03b |
1:5 |
0.3610 |
1.3998 |
6.9992 |
|
| PSU-03c |
1:5 |
0.5301 |
1.7304 |
8.6518 |
|
| PSU-04a |
1:2 |
0.3801 |
1.4372 |
2.8743 |
8.1856 |
| PSU-04b |
1:5 |
1.1546 |
2.9508 |
14.7540 |
|
| PSU-04c |
1:2 |
1.4174 |
3.4643 |
6.9286 |
|
| PSU-05a |
1:2 |
0.5677 |
1.8037 |
3.6074 |
3.0959 |
| PSU-05b |
1:2 |
0.7035 |
2.0691 |
4.1382 |
|
| PSU-05c |
1a |
0.4338 |
1.5420 |
1.5420 |
|
| PSU-06a |
1:10 |
0.6948 |
2.0523 |
20.5226 |
12.7650 |
| PSU-06b |
1:5 |
0.5606 |
1.7899 |
8.9493 |
|
| PSU-06c |
1:5 |
0.5476 |
1.7646 |
8.8230 |
|
|
| aThe original extract of this sample was used for analysis without dilution since the
amount of amylase in dilution was too low to be detected by UV-VIS |
| Table 4: Estimated concentrations of salivary amylase in the human saliva extracts
from six individuals taken at three different times of the same day. |
