Sample ID Dilution Absorbance of Dilution Estimated Conc. of Dilution (ppm) Conc. of the extract Average Concentration  of each donor
PSU-01a 1:2 0.8090 2.2490 4.4980 5.2875
PSU-01b 1:2 1.5844 3.7467 7.4934  
PSU-01c 1:2 0.6468 1.9356 3.8712  
PSU-02a 1:10 0.2942 1.2693 12.6933 20.6532
PSU-02b 1:10 1.1577 2.9567 29.5673  
PSU-02c 1:10 0.6527 1.9699 19.6990  
PSU-03a 1:10 0.6867 2.0363 20.3632 12.0047
PSU-03b 1:5 0.3610 1.3998 6.9992  
PSU-03c 1:5 0.5301 1.7304 8.6518  
PSU-04a 1:2 0.3801 1.4372 2.8743 8.1856
PSU-04b 1:5 1.1546 2.9508 14.7540  
PSU-04c 1:2 1.4174 3.4643 6.9286  
PSU-05a 1:2 0.5677 1.8037 3.6074 3.0959
PSU-05b 1:2 0.7035 2.0691 4.1382  
PSU-05c 1a 0.4338 1.5420 1.5420  
PSU-06a 1:10 0.6948 2.0523 20.5226 12.7650
PSU-06b 1:5 0.5606 1.7899 8.9493  
PSU-06c 1:5 0.5476 1.7646 8.8230  
aThe original extract of this sample was used for analysis without dilution since the amount of amylase in dilution was too low to be detected by UV-VIS
Table 4: Estimated concentrations of salivary amylase in the human saliva extracts from six individuals taken at three different times of the same day.