Column: PhenomenexKintex  C-18  
Dimensions: 5 µ, 100 A, 150 mm x 4.6 mm  
Mobile Phase:  0.1% acetic acid (v/v) in H2O (Eluent A)  
  0.1% acetic acit (v/v) in MeOH (Eluent B)  
Flow: 1 mL/min  
Gradient: 0% B to 10% B 0-8 min
  10% B to 60% B 8-15 min
  60% B to 70% B 15-20 min
  70% B to 80% B 20-25 min
  80% B 25-30 min
  80% B to 5% B 30-35 min
Injection Vol. 20 μL  
Column Temp: 35°C  
Sample Tray: 4°C  
Table 2: Chromatographic conditions.