Samples Run in Duplicate RapidHIT™ Traditional Method Volume of Blood (µL) Volume of Blood (µL) Average Quantity of DNA (ng) M1 and F1 1.0 1.0 1.2 M1 and F1 0.5 0.5 0.38 M1 and F1 0.25 0.25 0.28 M1 and F1 0.125 0.125 0.18 M1 and F1 0.0625 0.0625 0.15

Table 1: Volumes and quantity of DNA from diluted blood samples for sensitivity study. Five different volumes of blood, ranging from 1.0-0.0625 μL from M1 and F1 samples, run in duplicates using the RapidHIT™ System and the traditional method. The average quantity of DNA using the traditional method ranged from 1.2 to 0.15 ng.