Samples Run in Duplicate RapidHITTM Traditional Method Volume of Saliva (µL) Volume of Saliva (µL) Average Quantity of DNA (ng) Donor 1 and Donor 2 50.0 50.0 977.0 Donor 1 and Donor 2 40.0 40.0 678.8 Donor 1 and Donor 2 30.0 30.0 393.3 Donor 1 and Donor 2 20.0 20.0 65.6 Donor 1 and Donor 2 10.0 10.0 33.3

Table 2: Volumes and quantity of DNA from saliva samples for sensitivity study. Five different volumes of saliva, ranging from 50.0-10.0 μL from Donor 1 and Donor 2, run in duplicates using RapidHIT™ and the traditional method. The average quantity of DNA using the traditional method ranged from 977.0 to 33.3 ng.