Sample Short Name Experimental Design Experiment 1 Experiment 2 Experiment 3 (RapidHITTM and Traditional Method) Conditions Duplicate Run Donor 1 Stainless-steel spoon 24°C 48°C 48°C+43% Humidity Yes Donor 1 Plastic fork 24°C 48°C 48°C+43% Humidity Yes Donor 1 Plastic spoon 24°C 48°C 48°C+43% Humidity Yes Donor 1 Gum 24°C 48°C 48°C+43% Humidity Yes Donor 1 Stamp 24°C 48°C 48°C+43% Humidity Yes Donor 1 Stone 24°C NT* NT NT Donor 1 Straw 24°C NT NT NT Donor 1 Envelope 24°C NT NT NT Donor 1 Bottle 24°C NT NT NT Donor 1 Oil paint 24°C NT NT NT Donor 2 Stainless-steel spoon NT NT 48°C+43% Humidity Yes Donor 2 Plastic fork NT NT 48°C+43% Humidity Yes Donor 2 Plastic spoon NT NT 48°C+43% Humidity Yes Donor 2 Gum NT NT 48°C+43% Humidity Yes Donor 2 Stamp NT NT 48°C+43% Humidity Yes

Table 6: Experiments used to test the effect of heat and humidity on saliva Undefined amounts of saliva from Donor 1 and Donor 2 deposited on different substrates and subjected to three different conditions which included room temperature, dry heat and heat and humidity factors. Samples were run in duplicate in Experiment 3, where indicated. Both the RapidHIT™ System and the traditional method were used in Experiment 3. *NT = Not Tested