Experiment 4 (3.0 μL Blood) Sample  Substrates Collection Method Conditions RapidHITTM and Traditional Method Duplicate Run  F2 Synthetic leather 1.0 cm2 Cut 48°C+43% Humidity Yes F2 Denim jeans 1.0 cm2 Cut 48°C+43% Humidity Yes  F2 Branch Swabbed 48°C+43% Humidity NT  F2 Water paint 1.0 cm2 Cut 48°C+43% Humidity NT  F2 Plastic bag 1.0 cm2 Cut 48°C+43% Humidity NT M1 Unfinished wood Swabbed 48°C+43% Humidity Yes M1 Carpet fibers 3 Fibers Cut 48°C+43% Humidity Yes M1 Scarf 1.0 cm2 Cut 48°C+43% Humidity Yes M1 Laminated flooring Swabbed 48°C+43% Humidity NT M1 Kimwipe 1.0 cm2 Cut 48°C+43% Humidity NT F1 Synthetic leather 1.0 cm2 Cut 48°C+43% Humidity Yes F1 Denim jeans 1.0 cm2 Cut 48°C+43% Humidity Yes F1 Unfinished wood Swabbed 48°C+43% Humidity Yes F1 Carpet fibers 3 Fibers Cut 48°C+43% Humidity Yes F1 Scarf 1.0 cm2 Cut 48°C+43% Humidity Yes

Table 7: Experiment 4 used to test the effect of heat and humidity on 3.0 μL of blood F2, F1 and M1 blood samples (3.0 μL) deposited on various substrates and subjected to heat and humidity conditions. As indicated, ten of these samples were run in duplicate for reproducibility study. Both the RapidHIT™ System and the traditional method were used in this experiment.