Organism #  clones sequenced (expired lot)a PCR (expired lot)b #  clones sequenced (new lot) PCR (new lot)
Acinetobacter baumannii CP001172 5 + 0 -
Bacillus cereus CP000227 3 + 2 +
Bacillus licheniformis EF685205 15 + 58 +
Enterococcus faecium GQ405286 94 + 0 -
Brevibacillus choshinensis AB038650 0 + 2 +
Bacillus subtilis 0 - 38 +

a117 cpn60 isolates from the expired Product A and 100 cpn60 isolates from the new lot were sequenced. Bacterial sequences were determined by BLAST analysis using NCBI or against the cpn60 database (http://www.cpndb.ca/cpnDB/home.php) (Hill et al, 2004)
bPCR represents a positive signal against total extracted consortia DNA using cpn60 primers specific for that species
Table 5: Consortia composition by cpn60 isolate sequencing.