Figure 1: rAAV transduction was increased by isoproterenol (ISO) in vitro. (A) H9C2 cells were transduced by 1000 vector genomes/cell of rAAV with or without preincubation using ISO. Twenty-four hours after infection, the cells were stained for β-gal activity. The scale bar shows 200µM. (B) After incubation with indicated concentrations of ISO, the number of β-gal positive cells were counted. (C) Rat neonatal cardiomyocytes (CM) were transduced with rAAVMHC- lacZ vector with or without preincubation of using ISO. Twenty-four hours after infection, the CM were immunostained using an anti tropomyosin antibody following β-gal staining. The scale bar shows 40µM. (D) The number of β-gal positive CM were enumerated. Data are meanąSEM, *, p<0.05 (n=5) vs. PBS control.