Figure 4
Figure 4: Inhibition of calcineurin suppressed rAAV ds DNA formation. (A) Map of rAAV vector identifying EcoRI and SacI restriction enzyme digestion sites and the predicted sizes of ds DNA following digestion probed as shown. (B) Southern blot following EcoRI digestion (C) Alkaline-gel Southern blot following SacI digestion. ISO increased the relative amount of ds DNA compared with PBS control, and pretreatment with CNIP blocked this increase in H9C2 cells. The lane labelled ‘Posi’ in (B) and (C) was pasted from another region of the blot.