Figure 2: Nucleofection significantly improves gene transfer to B cells in comparison to conventional methods in different mouse strains. (a). Stimulated B cells isolated from C3H/HeOuJ mice showed efficient gene transfer by nucleofection. Lipofectamine had the lowest viability rates, while retroviral and adenoviral transduction caused the least damage to B cells. (b). B cells from C57BL/6 B mice showed similar gene transfer efficiency and viability for the respective vector system and protocol. Data for graphs (a) and (b) are average ±SEM of 3 experiments per strain using 5 replicates of gene transfer per experiment. (c). For nucleofection, increasing plasmid DNA doses reduced B cell viability (C3H/HeOuJ B cells, 5 replicates per plasmid dose).