Figure 1: Structure of helper-dependent adenoviral vectors expressing APOE3. A. Schematic presentation of helper-dependent adenoviral vectors (HDAd) containing phosphoenolpyruvate carboxykinase (PEPCK) promoter on C4HSU backbone. L-ITR and R-ITR indicate left and right adenovirus (Ad) inverted terminal repeat sequence, respectively; Ψ, Ad packaging signal; PEPCKpr, PEPCK promoter; AI intron, human apolipoprotein AI intron, hGH polyA, human growth hormone polyadenylation signal; WPRE, Woodchuck hepatitis virus post-transcriptional regulatory element; LCR, human APOE gene liver control region. B. HDAd containing human APOE gene promoter and liver specific enhancer, LCR. In HDAd-E-E3, the part of exon 2 to the part of exon 4 was removed and replaced with APOE3 cDNA. HDAd-gE3 contains APOE3 5’ flanking region as well as all exons. C. HDAd containing human APOAI promoter and 3’ flanking region. In HDAd-AI-E3, the part of APOAI exon 2 to the part of exon 4 was removed and replaced with APOE3 cDNA. D. HDAd containing human APOE3 gene and LCR on pΔ21 backbone. HPRT, intron region of human genomic HPRT; C346, cosmid C346 human genomic stuffer sequence.