Figure 6: Plasma cholesterol levels in Ldlr−/− mice after hepatic LDLR gene transfer. 6-8 week old Ldlr−/− mice on high cholesterol diet were treated with a single injection of HDAd expressing LDLR and plasma cholesterol was measured at indicated time. All data are presented as mean ± SD. A. Effects of expression cassette and Ad E4 promoter. HDAd vectors are on pΔ21 or pΔ28 backbone. *p<0.01, **p<0.001 vs. PBS. n=5/group. At 12 weeks, plasma cholesterol levels in the HDAd-P-LDLR group were no longer different from those of the PBS group. B. Incorporation of LDLR gene structure into HDAd. n=3