Figure 7: Comparison of single gene therapy and combined gene therapy on plasma cholesterol. A. Experimental design. 6-8 week old female Ldlr−/− mice were fed a diet containing 0.2% (w/w) cholesterol and 10% (v/w) coconut oil for 36 weeks to induce atherosclerosis. Mice were separated in 5 groups. One group (baseline) was sacrificed and other 4 groups received single i.v. injection of HDAd vector. A high cholesterol diet was maintained throughout experiment. n=13 (baseline), 13 (HDAd-0), 14 (HDAd-LDLR), 12 (HDAd-AI) and 15 (HDAd-LDLR + HDAd-AI). B. Plasma cholesterol levels. *p<0.01, **p<0.001 vs. HDAd-0. C. FPLC analysis. Plasma collected 28 weeks after vector treatment. 0.2 ml of pooled plasma was separated with FPLC and cholesterol was determined. VLDL: very low density lipoprotein; IDL/ LDL: intermediate-density-lipoprotein/low density lipoprotein; HDL: high density lipoprotein.