Figure 3: The kinetic activity measurement of TACI and BAFF or its doman mutant. Affinity measurement on the sensor surface using ForteBio: cruve of the experimental data corresponding to phases of association and dissociation of proteins (Trx, rhBAFF, rhCBAFF mutants) at various concentrations on the TACI-Ig anchored to the sensor chip. Starting from the bottom the protein concentrations are: 900, 600, 300 μg/mL. These curves were used to determine KD, Kon and Koff.
Trx 、rhBAFF 、M1 、M2 、M3 and M4 (Figure 2).