Table 1

Variant	K_D (M)	K_on (1/Ms)	K_off (1/s)	R²
rhBAFF	3.63E-08	9.64E+02	3.50E-05	0.872721
Trx	---	---	---	0*
M1	3.18E-07	2.93E+02	9.34E-05	0.951368
M2	1.17E-06	4.18E+02	4.88E-04	0.914619
M3	5.33E-07	4.60E+02	2.45E-04	0.959939
M4	---	---	---	0*

Table 1: The binding kinetics of the BAFF receptor TACI to BAFF and BAFF mutant using the ForteBio system Off-rate kinetics (k_off) were measured by saturating the chip with TACI and then monitoring dissociation after switching to buffer. On-rate kinetics (k_on) was measured using 3 kinds of dilutions of BAFF or BAFF mutant. K_D, the equilibrium dissociation constant, was calculated as K_off/ K_on. Trx、rhBAFF 、M1、M2、M3 and M4: see Figure 2.
The bar (---) means the data is too limited to be analyzed.
*Curve equations could not be established to fit with the experiment data and fitting accuracy (R²) showed only zero.