Figure 1: In vitro infection of GL261 mouse glioma cells with recombinant rAAV2-WT-CBA-RFP, rAAV2-TRP-CBA-RFP and rAAV-ShH19-CBA-RFP viruses. A. Fluorescent microscope images of GL261 cells in 12-well plates infected with rAAV2-WT-CBA-RFP, rAAV2-TRP-CBA-RFP and rAAV-ShH19-CBA-RFP viruses at the MOI 10,000 (Original magnification: 100x). AAV2-TRP is AAV2 capsid with Y(444, 500, 730)F tyrosine mutations (“triple mutant”). B. Representative low cytometry results. C. Summary of the flow cytometry data for three different MOIs. Data are average ± SD for triplicate measurements.