Figure 3: In vivo transduction of neurons in proximity to GL261 tumors. A. Confocal microscopy images of representative brain sections from gliomabearing C57BL/6 mice receiving rAAV2-TRP-CBA-RFP (upper panels) or rAAV-ShH19-CBA-RFP (lower panels) vectors injected directly into GL261- GFP tumor. AAV2-TRP is AAV2 capsid with Y(444, 500, 730)F tyrosine mutations (“triple mutant”). Green signal is GFP stably expressed in glioma, red signal is RFP expressed from vector. Original magnification: 200x. B. Higher magnification image of cells with neuron morphology transduced with rAAVShH19- CBA-RFP as a representative example of gene transfer to neurons. Original magnification: 300x.