Author Cells lines Design Result Transport Ref
Walther W. et al. MCF-7
In Vitro
In Vivo
Effective against
(overexpressing claudin-3-, -4-)
MichaelsenS. al. GLC-14, GLC-16,
GLC-19, NCI-H69,
H69-VP, H69-CPR,
In Vitro
In Vivo
Effective both in chemosensitive andchemoresistant celllines INSM1 promoter-driven SG [47]
Mader R.M. et al. CCL227 (with low and Intermediate phenotypes) In Vitro Effective with 100%Activation Adenoviral cosmids [8]
Bondanza A. et al. Leukemia (mouse) In VitroIn Vivo Effective withIL-7 receptorexpression(HA-1-, H-Y-) Herpes simplex virusThymidine kinase (tk) [45]
Xu Y. et al. Lewis Lung Cancer
In VitroIn Vivo Combination IL-12 and suicide genetherapy enhances the antitumor effectas a factor modifying the tumor microenvironment AdCMV(-), AdhTERTHRP, AdCMVmIL-12  [46]
Sia KC. et al. HCC 26-1004 In VitroIn Vivo Effective HSV-1 amplicon viral vector and 5-FU administration HSV-1 amplicon viral Vector coupled with yCD [9]
Li S. et al. C17.2 NSC line In VitroIn Vivo ATRA enhanced the HSV-tk/GCV HSVtk/GCV [30]
FinocchiaroM.E.L. et al. sarcoma In Vivo Effective MicroenvironmentControl and Distant metastasis Lipid-complexed plasmid Bearing IFN-β and suicide genes co-administered with ganciclovir (ISG) [42]
Leng A. et. al. Human colon carcinoma(Lovo) cell line In VitroIn Vivo Anti-VEGF-A-Suicide gene therapy 5-FC, CPNP-shVEGF-CDTK [43]
Liu T. et. al. SGC7901 human gastricCancer cell line In vitroIn Vivo Anti-VEGF-Suicide gene therapy 5-FC, triple gene vector ExpressingVEGF-shRNA and fusion suicide geneyCDglyTK delivered by CPNPs [44]
Finzi et. al. Human HT29 and murineDHDK12 pro-b In VitroIn Vivo MTX, aphidicolinandara-C. The rate of apoptosis increased two-fold in MTX-treated DHDK12 cells after treatment with GCV. HSVtk-GCV [25]
Niu H. et. al. VX2 liver cancer In Vivo Effective with lipiodolembolism and WTp53 TK/CD plus intraperitonealInjection of GCV at 100mg/(kg.d)and 5-FC at 500mg/(kg.d) [26]
MarukawaY. et. al. HCC In vitroIn Vivo Effective Mac-1, CD4,CD8a-positive and TNF increase -HSV-tk/GCV and MCP-1-rAd harboring human MCP-1and the membrane-spanning domain of the tumor cell surface [27]
Kosaka H. t. al. 9L rat glioma cells and 293 cells In vitroIn Vivo MSC-EGFP or MSC-CD-5-FCresulted in significant prolongation of survival AdexCAEGFPAdexCACD   [39]
Schmidt al. Head and Neck squamous carcinoma cell line FADU In Vitro Effective  with deletion Mutant of ETA as a Targetgene Gene Switch System [41]
Cottin S. et. al. Glioblastoma In Vitro Effective against Cx43 cytoplasmic localization Lentiviral delivery of HSV-tk/GCV  [40]
Kakinoki K. et. al. HCC In vitroIn Vivo Effective against metastasis and controlof microenvironment CCL2/MCP-1HSV-tk/GCV [49]
Sun X. et. al. R3327-AT rat prostateCarcinoma cells In VitroIn Vivo Effective against hypoxic cells Bifunctionalcytosine deaminase (CD) anduracil phosphoribosyltransferase(UPRT) with 5-FC and radiotherapy [48]
Amano S. et. al. C6 glioma cells In VitroIn Vivo Safety evaluation of theStem cell therapy in braintissue Rat MSCtk/GCV [65]
Zhao Y. et. al. U87 glioma and H4 cells In VitroIn Vivo Effective as cellular Vehicle for targeted suicide genetherapy Tumor-tropic neural stem cells, HSV-tk/GCV [38]
Wang C. et. al. NCI-H460-GFP cells In VitroIn Vivo Effective brain metastasistreatment NSC line expressingCD and TK  [37]
Yin X. et. al. Bladder cancer withN-methyl-nitrosoureaperfusion In VitroIn Vivo Effective both in extrisincand intrisincpapoptosispathways BI-HSV-tk/GCV [36]
Leveille S. et. al. Prostate PC3, Breast MCF7,TSA mammary Adenocarcinoma, B-Lymphoma Karpas-422,Melanoma B16-F10 In VitroIn Vivo Synergistic effect of (VSV)-ΜΔ51 and 5FC Combination (VSV)-ΜΔ51-Expressing(CD::UPRT)-5FC    [85]
Cramer F. et. al. SCLC: GLC16, DMS53 andNCI-H69  and NSCLCcancer lines: H1299 and A549 In Vitro Improved plasmidnucleardelivery NFnB-DTS in anYCD-YUPRT (SCD) [28]
Li J. et. al. SGC7901 human gastric cancer cell line In Vitro Effective Cell apoptosis YCDglyTK and Htert with CPNPs /Novel vector pcDNA3.1(-)hTERT-ShRNA / YCDglyTK   77
Duan X. et. al. C-26 In VitroIn Vivo DMP Delivered Survivin-T34A gene DMP/S-T34A) which induced apoptosis DOTAP and MPEG-PCL hybrid micelles (DMP)   [50]
Tang Q et. al. Human hepatic cell line (HepG2) In VitroIn Vivo Survival time and apoptosis Intratumorally injected KDR-TK, AFP-TK and microbubbleContrast agent with 5-FC and GCV [51]
ZarogoulidisP. et. al. Lewis lung cancer,SCLC, NSCLC patients Animals
Survival and  malignant pleural effusion control with higherefficiency observed for SCLC. Ad.CD+5-FC [6]
Yi B. et. al. Review Review Review Review [35]
Qiu  Y. et. al. A549, 16HBE, SPC-A-1 AndNCI-H520 In Vitro Specific CA-positive Target gene expression CEA promoter and double suicide genes TK and CD.pCEA-TK/CD   [29]
Won Y. et. al. C6, U87, F98 and 9L In VitroIn Vivo Tumor growth Suppressionand locomotor functionmaintenance rPOA/HSV-tk/GCV [32]
Akerstrom V. et. al. Neuroendocrine tumors:NCI-H69, NCI-H1155, NCI-H727, DMS53, U87MG,IMR-32, S-N-SH, SK-N-BE(2),Y79, WERI-Rb1, HeLa, ANC-1,BEAS, RIN, D283 Med, HepG2 In VitroIn Vivo Enhanced antitumor activity over the RSV control INSM1 promoter, HSV-tk to generate Ad-K5 virus     [31]
Lu M. et. al. Prostate Human Initiatedand recruiting at the time of publication Replication-Competent Adenovirus- mediated suicide gene therapy [33]
Ma S. et. al. MCF-7 and MDA-MB-231Breast cell lines In VitroIn Vivo Effective antitumor control Drosophila melanogaster(Dm-dNK)  [34]
Preuss E. et. al. G62 human glioblastoma cellLine, A549 human lung Carcinoma, SW620 human Colorectal adenocarcinomaCell line and IPC298 HumanMelanoma cell line In VitroIn Vivo Continuous complete remission TK.007 novel suicide gene [95]
Ahn Y. et. al. CT26 murine colon adenocarcinoma cells andAGS human gastric adenocarcinoma cells In VitroIn Vivo Effective combination Suicide immune therapy shRNA-lentivirusand  [24]
Gruber C. et. al. SCC In VitroIn Vivo Efficient transfection of RDEB SCC SLO=PTM [96]
Xiong J. et. al. HeLa human cervical cancer Cells, A549 human lung Adenocarcinoma cells,MHCC97 human hepatomaCarcinoma cells and hELNormal human embryonivLung cells In Vitro Combinational cancer Promoter responsive to irradiation Chimeric hTERT promoters, CArG [82]
Luo X. et. al. SGC7901 human gastricCancer cell lines In VitroIn Vivo Higher efficiency withdouble suicide gene therapy CD/TK Double suicide gene therapyAd-survivin/GFP andAd-survivin/CD/TK [59]
Freytag S. O. et. al. Prostate cancer Human Transgene expression up to 3 weeks, PSA decline, Acute urinary and gastrointestinal toxicities Cytosine deaminasa(CD)/herpes simplexvirus thymidine kinase (HSV-1 TK) and 3D-CRT [92]
Pandha H. S. et. al.  Breast cancer Human Efficient selectivity against erb-2 Therapeutic cassette that contains the Escherichia colicytosinedeaminase gene drivan by the tumor-specific erb-2 promoter [57]
Li N. et. al. HCC cancer Human Recurrence free survival Adjuvant ADV-TK [91]
Voges J. et. al. Glioblastoma Human Inhomogeneity of tissue formulation distribution HSV-1-tk liposomal vector [88]
Nasu Y. et. al.  Prostate Human No serum cytokine changes after treatment,decreased PSA values,Increased CD8+/HLA-DR+This study confirmed thesafety profile at the surrogate markerof HSV-tk gene therapy. Ad.HSV-tk/GCV [89]
Rainov N.G. et. al. Glioblastoma Human Surgical resection and Radiotherapy or standardtherapy plus adjuvant gene therapy during surgery. Progression-free median survival in the gene group was 180 days compared with 183 days of control group RV-HSV-tk/GCV [76]
Xu F. et. al. Head and Neck Human Local response Intratumoral RV-HSV-tk/GCV [90]
Nemunaitis al.  Refractory cancer patients Human Salmonella bacterium can be utilized as a delivery vehicle of thecytosine deaminase gene to malignant tissue with low dose 3 x 107 CFU/m2 efficiently. TAPET-CD   [94]
Freytag S. al. Pancreas Human Augments radiotherapytreatment of pancreaticcancer without excessivetoxicity  Ad5-yCD/mutTKSR39rep-ADPHSV-1 TK SR39 [93]
INSM1;insulinoma-associated 1 gene, IFN-β; Interferon-β, GCV;ganciclovir, CEA;Carcinoembryonic antigen, ELISA;Enzyme-linked immuno sorbent assay, IL-7; Interleukin-7, wtCPE; wild type Clostridium perfringens enterotoxin, optCPE; translation-optimisedClostridium perfringens enterotoxin, IL-12; interleukin-12, HSV-1; herpes simplex virus-1, 5-FC; 5-Fluorocytosine, VEGF; vascular endothelial growth factor, CPNPs;bioresorbable calcium phosphate nanoparticles, HSV-tk;herpes simplex virusthymidine kinase, yCDglyTK; fusion gene therapy of cytosine deaminase and thymidine kinase, Mac-1;Macrophage-1 antigen, CD4+; T lymphocytes, referring to those that carry the CD4 antigen, CD8+; T lymphocytes, referring to those that carry the CD8 antigen, CCl2;chemokine (C-C motif) ligand 2 [Homo sapiens (human), rAd; recombinant adenovirus, MCP-1;Monocyte chemoattractant protein-1, ETA; ETA receprtors, WTp53; wild type p53, Cx43;integral membrane protein of the connexin family, alpha-type (group II) subfamily, MSC;mesenchymal stem cells, AdexCAEGFP; MSC- adenovirus carrying either enhanced green fluorescent protein gene, AdexCACD; MSC- cytosine deaminase gene, UPRT; uracil phosphoribosyltransferase, NSCs; neural stem cells, hTERT; human telomerase reverse transcriptase, HRP; expressing horseradich peroxidase, IAA; idole-3-acetic acid, CArG;Smooth muscle alpha-actin CArG elements, BI;Bifidobacteriuminfantis, VSV; vesicular stomatitis virus, NFnB; nuclear factor B, DOTAP;N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl sulfate,MPEG-PCL;monomethoxy poly(ethylene glycol)–poly(3-caprolactone , DMP; DOTAP–MPEG–PCL, KDR; kinase insert domain receptor, AFP;α-fetoprotein, rPOA; poly (oligo-D-arginine), DM;Drosophila melanogaster,PET; positronemission tomography,;adenoviruse 5 harboring the herpes simplex virus thymidine kinase gene,SLO=PTM; toxin Streptolysin O-3` pre-trans-splicing molecules, RDEB-SCC; recessive dystrophic epidermolysisbullosa squamous cell carcinoma, PTM screen; 3` pre-trans-splicing molecules, PSA; prostate specific antigen, GDEPT; gene-directed enzyme pro-drug therapy, TAPET-CD; pCVD442-msb B-VNP20009
Table 1: Suicide Gene Therapy Studies