Figure 5: Monoubiquitination assays. Western blotting of the FANCD2 protein in whole cell lysate of EUFA868 cells infected with either the FANCL or FANCLΔ4 cDNA, or not infected (ø). Cells were treated or not with 100ng/ ml of MMC for 16 hours. FANCD2 associated with an ubiquitin is found at 162 kDa whereas FANCD2 alone weights 155 kDa. Calnexin is used as loading control.