Figure 6: Survival assay and cell cycle profiles. (A) MMC-induced cell death. Differential survival of EUFA868 (diamond), EUFA868/pMSCVneo (circle), EUFA868/FANCL (triangle) and EUFA868/FANCCΔ7 (square) cells after 5 days of incubation in escalating concentrations of MMC. The fraction of surviving cells was measured by the Alamar Blue assay. Untreated cells are shown in the right panel. (B) FACs analysis of the EUFA868 FANCL-deficient cell line infected with FANCL cDNA, FANCLΔ4 cDNA or not infected at all. Cells were treated with MMC (50 ng/ml) for 24 hours prior to FACs analysis.