Figure 2: Ribosomal fractions. Polysome analysis of the FANCCΔ7 alternative transcript in T47D cells. The square line is the data acquired from the -EDTA sample whereas the circle line is the data from the + EDTA sample. Fractions represent the 15-45% sucrose gradient used to separate by weight the mRNA and the ribosomal units associated. Greater the fractions are, heavier the mRNA and ribosomal complexes (polyribosome) meaning more association between ribosomal units and mRNA.