Primer sequence of p53 R72P G>C (rs1042522) Product size Annealing Tempt
Arg72 allele F -5’-TCCCCCTTCCCGTCCCAA-3’ 141 bp 55°C
R- 5’-CTGCTGCAGGGGCCACGC-3’
Pro72allele F-5’-GTCCTCTGACTGCTGTTATCACCCATCTAC-3’ 177 bp 60°C
R-5’- GGGATACGGCCAGGCATTGAAGTCTC-3’
Table 1: Primer sequence for ASO-PCR used for p53 codon 72 polymorphism.