| |
CELL LINE |
| NORMAL |
BRCA1 |
BRCA2 |
| GM00893 |
GM05423 |
GM13705 |
GM14090 |
GM16105 |
GM14170 |
GM14622 |
GM14805 |
| Olaparib |
+ |
− |
+ |
− |
+ |
− |
+ |
− |
+ |
+ |
+ |
+ |
| SF2 Mean (%) |
83.2 |
89.5 |
86.9 |
82.3 |
75.3 |
84.1 |
78.1 |
89.4 |
43.5 |
79 |
49.3 |
87.1 |
| SF2 Standard Error |
10.6 |
7.5 |
7.1 |
15 |
7.3 |
8.2 |
2.2 |
2.2 |
1.4 |
4 |
8.2 |
6.5 |
Table 2 shows the surviving fraction following exposure to 2 Gy gamma radiation (SF2). For each cell line the data are derived from a minimum of three independent
experiments. The means SF2 for each cell lines with or without incubation with 5 μM Olaparib is shown together with the standard error of the mean SF2. Statistical analysis
using a Student’s unpaired T-test show that there are no significant differences in the SF2 values when the normal lymphoblastoid cells are compared with either the
BRCA1 and BRCA2 heterozygous cells. However, BRCA1 heterozygous cells are significantly more sensitive to ionising radiation in the presence of the PARP-1 inhibitor
Olaparib (P =0.02) |
