CELL LINE
NORMAL BRCA1 BRCA2
GM00893 GM05423 GM13705 GM14090 GM16105 GM14170 GM14622 GM14805
Olaparib + + + + + + + +
SF2 Mean (%) 83.2 89.5 86.9 82.3 75.3 84.1 78.1 89.4 43.5 79 49.3 87.1
SF2 Standard Error 10.6 7.5 7.1 15 7.3 8.2 2.2 2.2 1.4 4 8.2 6.5
Table 2 shows the surviving fraction following exposure to 2 Gy gamma radiation (SF2). For each cell line the data are derived from a minimum of three independent experiments. The means SF2 for each cell lines with or without incubation with 5 μM Olaparib is shown together with the standard error of the mean SF2. Statistical analysis using a Student’s unpaired T-test show that there are no significant differences in the SF2 values when the normal lymphoblastoid cells are compared with either the BRCA1 and BRCA2 heterozygous cells. However, BRCA1 heterozygous cells are significantly more sensitive to ionising radiation in the presence of the PARP-1 inhibitor Olaparib (P =0.02)
Table 2: Surviving fraction at 2 Gray (SF2) of each cell line irradiated with gamma radiation.