Figure 1: The DNA constructs with the coding sequences for human recombinant DNase1, DNase1L3, DNase2, DFFB (hrDNases) were delivered by anti-EGFRvIII (DEF) and anti-EGFR (GHI) antibody guided vectors into the nuclei of EGFRvIII + or EGFR+ over-expressing ovarian cancer cells from the patients’ ascites. They were labeled with the anti-dsDNA (D,G) or anti-phosphatidylserine (F,I) and imaged with phase contrast (E,H) or Ploem’s epifluorescence (D,F,G,I). Non-transduced EGFRvIII+ ovarian cancer cells from ascites stained with bisbenzimide (A) and labeled with anti-EGFRvIII antibody (C), were imaged with phase contrast (B) or Ploem’s fluorescence (A,C) as the controls. Transgenic expression and intranuclear targeting of the hrDNases in ovarian cancer cells resulted in collapse of the chromatin architecture, as highlighted after labeling with anti-dsDNA fluorescent antibodies (E,G), as well as externalization of phospatidylserine as highlighted after labeling with anti-PS fluorescent antibodies (F,I).